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Description
The Viroreal Kit EMCV is a diagnostic tool intended for veterinary use to detect the Encephalomyocarditis virus (EMCV). This virus is known to cause encephalomyocarditis and reproductive diseases in swine and other mammals. The kit utilizes a one-step reverse transcription real-time PCR (RT-PCR) method to identify the RNA of the virus from various biological samples. EMCV can lead to conditions such as myocarditis, encephalitis, and reproductive failure in affected animals.
Diagnostic Test: This is a ViroReal® Kit designed for the detection of Encephalomyocarditis virus (EMCV) RNA. Detection Method: It employs a one-step reverse transcription RT real-time PCR technique for the identification of the virus. Target Pathogen: The kit is specifically designed to detect the 3´UTR RNA of EMCV, a virus that can cause disease in swine, primates, elephants, and other mammals. Comprehensive Kit: The kit contains an assay mix, a positive control, and an internal positive control (IPC) to help ensure the accuracy of the results. Broad Compatibility: It is developed to be compatible with a range of established standard real-time PCR platforms.
Q: What is the Viroreal Kit EMCV 1's?
A: The Viroreal Kit EMCV 1's is a real-time RT-PCR test designed for the in-vitro detection of Encephalomyocarditis virus (EMCV) RNA. It is intended for veterinary use only.
Q: What is Encephalomyocarditis virus (EMCV)?
A: EMCV is a single-stranded RNA virus that can cause encephalomyocarditis and reproductive issues in various mammals, particularly swine. It can lead to conditions like myocarditis, encephalitis, and reproductive failure in affected animals. Rodents are considered the primary reservoir for the virus.
Q: Who should use this kit?
A: This diagnostic kit is intended for use by veterinary professionals to detect the presence of EMCV in susceptible animals like pigs, primates, elephants, and rodents.
Q: How does the kit work?
A: The kit utilizes a one-step reverse transcription real-time PCR (RT-PCR) method to detect the 3´UTR RNA of the Encephalomyocarditis virus. It includes primers and probes designed for sensitive and specific detection, along with positive and internal controls to prevent false-negative results.
Q: What kind of samples can be tested with this kit?
A: The kit is used to detect EMCV RNA from samples purified from sources such as tissue biopsies, semen, and nasal swabs of animals.
The Viroreal Kit EMCV is a diagnostic tool intended for veterinary use to detect the Encephalomyocarditis virus (EMCV). This virus is known to cause encephalomyocarditis and reproductive diseases i...
Key Benefits
Aids in the detection of Encephalomyocarditis virus (EMCV), a pathogen that causes reproductive and cardiac diseases in various animals, primarily swine. The use of a one-step RT-PCR process allows for reverse transcription and the PCR amplification to be performed in a single reaction well, streamlining the diagnostic workflow. Includes an internal control system designed to prevent false-negative outcomes and verify the reaction's integrity. The primers and probe are carefully designed to provide sensitivity and specificity for the detection of EMCV RNA. The kit is optimized to handle potential PCR inhibitors that may be present in samples.
Aids in the detection of Encephalomyocarditis virus (EMCV), a pathogen that causes reproductive and cardiac diseases in various animals, primarily swine. The use of a one-step RT-PCR process allows...
Direction for Use/Dosage
This product is for veterinary use only and should be handled by trained professionals in a laboratory setting. The kit uses a one-step reverse transcription RT real-time PCR process. Reverse transcription and PCR amplification are performed in the same reaction well. Follow the detailed instructions provided in the product manual for sample preparation and use with a compatible real-time PCR instrument.
This product is for veterinary use only and should be handled by trained professionals in a laboratory setting. The kit uses a one-step reverse transcription RT real-time PCR process. Reverse trans...
Safety Information/Precaution
For veterinary use only. The test should be performed in a laboratory environment. It is advisable to wear appropriate personal protective equipment, such as disposable gloves, when handling samples and kit reagents. Follow standard laboratory procedures for handling biological specimens and for disposing of used materials.
For veterinary use only. The test should be performed in a laboratory environment. It is advisable to wear appropriate personal protective equipment, such as disposable gloves, when handling sample...
Other Information
Store the kit at -20°C; it is shipped on dry ice to maintain this temperature. The shelf life for RNA pathogen detection kits is typically 12 months. The kit is validated for use on platforms like the Applied Biosystems® 7500 System, LightCycler® 480, and Mx3005P® QPCR System, and is suitable for other standard real-time PCR instruments.
Store the kit at -20°C; it is shipped on dry ice to maintain this temperature. The shelf life for RNA pathogen detection kits is typically 12 months. The kit is validated for use on platforms like ...