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Description
The Bionote TB-Feron Elisa Plus Test Kit is a veterinary diagnostic tool for the qualitative detection of bovine Interferon-gamma (IFN-?). This sandwich ELISA (enzyme-linked immunosorbent assay) is designed to identify cell-mediated immunity against Mycobacterium bovis, the causative agent of bovine tuberculosis. The assay works by measuring the IFN-? produced by an animal's T-cells in response to specific TB antigens, serving as a marker for infection. It is intended for use with heparin-treated whole blood from cattle.
Bovine Tuberculosis Detection: Specifically designed for the diagnosis of Mycobacterium bovis (bTB) infection in cattle.
Interferon-Gamma Assay: Functions by detecting bovine IFN-?, a cytokine that is a specific marker in the cell-mediated immune response to TB infection.
ELISA Technology: Utilizes a sandwich ELISA method with monoclonal and polyclonal antibodies for the measurement of bovine IFN-?.
For Veterinary Use: This kit is intended for in-vitro diagnostic use in bovine (cattle) species only.
Sample Type: The test is performed using plasma from heparin-treated whole blood samples.
Q: What is the Bionote TB-Feron Elisa Plus Test Kit?
A: This is a sandwich ELISA (enzyme-linked immunosorbent assay) kit intended for the qualitative detection of interferon-gamma (IFN-?) in bovine (cattle) samples to aid in the diagnosis of bovine tuberculosis. It is designed for in-vitro diagnostic use by veterinary professionals.
Q: What is the principle of this test?
A: The test works by detecting a specific immune response. When whole blood from an animal infected with Mycobacterium bovis is stimulated with specific antigens in the kit, the animal's memory T-cells are prompted to produce interferon-gamma (IFN-?). The ELISA then measures the presence of this IFN-?, indicating an infection.
Q: What type of sample is required for this test?
A: The primary sample required is heparin-treated whole blood from bovine animals. The test is performed on the plasma separated from the stimulated whole blood.
Q: How should this test kit be stored?
A: The general storage temperature for the ELISA kit is between 2-8°C (36-46°F). It is important to follow the specific storage instructions for each component provided in the kit.
Q: Is this test affected by the BCG vaccination?
A: An advantage of interferon-gamma release assays (IGRAs) like this one is that they are generally not affected by a prior Bacille-Calmette Guerin (BCG) vaccination, which can cause false-positive results in traditional skin tests.
The Bionote TB-Feron Elisa Plus Test Kit is a veterinary diagnostic tool for the qualitative detection of bovine Interferon-gamma (IFN-?). This sandwich ELISA (enzyme-linked immunosorbent assay) is...
Key Benefits
Aids in the diagnosis of bovine tuberculosis by detecting the animal's cellular immune response.
Supports veterinarians in managing and controlling tuberculosis in cattle herds.
Provides a method for measuring cell-mediated immunity (CMI) in cattle after stimulating whole blood with antigens.
Aids in the diagnosis of bovine tuberculosis by detecting the animal's cellular immune response.
Supports veterinarians in managing and controlling tuberculosis in cattle herds.
Provides a ...
Direction for Use/Dosage
This test kit is for professional veterinary use only.
The assay involves collecting heparin-treated whole blood and cultivating it with the provided stimulating antigens (PPD B, PPD A) and a control (PBS).
After an incubation period, the plasma is separated.
The ELISA procedure is then performed using the antibody-coated microplate and other reagents to measure the amount of IFN-? in the plasma samples.
Results are determined by reading the optical density with an ELISA reader.
This test kit is for professional veterinary use only.
The assay involves collecting heparin-treated whole blood and cultivating it with the provided stimulating antigens (PPD B, PPD A) and a c...
Safety Information/Precaution
For in-vitro veterinary diagnostic use only.
Handle all blood samples as potentially infectious material and use disposable gloves during the procedure.
Do not use samples that are hemolyzed or appear contaminated.
Test results may be influenced by factors causing immunosuppression, such as recent dexamethasone treatment or calving within the last 4 weeks, which can lead to false-negative outcomes.
It is recommended to re-test animals under these conditions after a suitable period.
This kit is specifically for bovine IFN-? and its use on other species is not guaranteed.
For in-vitro veterinary diagnostic use only.
Handle all blood samples as potentially infectious material and use disposable gloves during the procedure.
Do not use samples that are hemolyze...
Other Information
Store the kit at a temperature of 2-8°C.
Do not freeze the components.
Use fresh samples for testing.
The kit includes components such as an antibody-coated microplate, stimulating antigens, controls, washing solution, enzyme conjugate, TMB substrate, and stop solution.
Store the kit at a temperature of 2-8°C.
Do not freeze the components.
Use fresh samples for testing.
The kit includes components such as an antibody-coated microplate, stimulating anti...