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Description
The Bionote BTB Ab ELISA 2.0 is a diagnostic test kit intended for veterinary use. It is designed for the qualitative detection of antibodies against Mycobacterium bovis, the bacterium that causes bovine tuberculosis, in cattle serum. This direct Enzyme-Linked Immunosorbent Assay (ELISA) serves as a tool for veterinarians and laboratories to screen cattle herds for exposure to the pathogen. The assay is developed for professional application and aids in the implementation of animal health and disease control programs.
Specific Antigen Use: The test utilizes microplates pre-coated with purified M. bovis antigen, allowing for the targeted detection of specific antibodies in bovine serum samples.
Herd Screening Tool: This serological test is suitable for the large-scale screening of cattle herds, helping to identify animals in the mid to late stages of bovine tuberculosis infection.
Inclusive Test Kit: The kit contains the necessary components to perform the assay, including an antigen-coated microplate, positive and negative controls, enzyme conjugate, washing solution, substrate, and stop solution.
Q: What is the Bionote BTB Ab ELISA 2.0 Test Kit used for?
A: This kit is a direct Enzyme-Linked Immunosorbent Assay (ELISA) for the qualitative detection of antibodies against Mycobacterium bovis, the causative agent of bovine tuberculosis, in bovine serum.
Q: Who is this test kit intended for?
A: This diagnostic tool is designed for use by veterinary professionals and laboratories for the screening and monitoring of bovine tuberculosis in cattle.
Q: What type of sample is required for this test?
A: The test is performed using serum samples from cattle.
Q: Does this kit require any specific equipment to use?
A: Yes, as an ELISA-based test, it requires standard laboratory equipment such as precision pipettes, a vortex mixer, and a 96-well plate reader (spectrophotometer) to perform the assay and read the results.
Q: How does this test contribute to managing bovine tuberculosis?
A: It serves as a tool for massive screening, especially for identifying cattle in the mid to late (anergy) stages of infection, and can be used in conjunction with other tests like the PPD skin test or IFN-? assays in eradication programs.
The Bionote BTB Ab ELISA 2.0 is a diagnostic test kit intended for veterinary use. It is designed for the qualitative detection of antibodies against Mycobacterium bovis, the bacterium that causes ...
Key Benefits
Assists in identifying cattle that have developed an antibody response to Mycobacterium bovis.
Functions as a component in herd health management strategies and bovine tuberculosis control initiatives.
Provides qualitative results regarding the presence of antibodies, contributing to a comprehensive diagnostic picture.
Assists in identifying cattle that have developed an antibody response to Mycobacterium bovis.
Functions as a component in herd health management strategies and bovine tuberculosis control init...
Direction for Use/Dosage
This test kit is intended for laboratory and professional veterinary use only. The general procedure is as follows:
1. An equal mixture of the serum sample and the M. Bovis antigen-HRP conjugate is prepared and added to the antigen-coated microplate wells.
2. The plate is incubated for 60 minutes at 37°C.
3. After incubation, the wells are washed to remove unbound materials.
4. A TMB substrate is added to the wells, and the plate is incubated again.
5. A stop solution is added to terminate the reaction, and the colorimetric result is read using a spectrophotometer.
Always follow the detailed instructions for use provided within the test kit.
This test kit is intended for laboratory and professional veterinary use only. The general procedure is as follows:
1. An equal mixture of the serum sample and the M. Bovis antigen-HRP conjugat...
Safety Information/Precaution
For in-vitro veterinary diagnostic use only.
The test must be administered and interpreted by a qualified veterinary professional.
A final diagnosis should not rely solely on the results of this test but should be made in conjunction with other laboratory tests and clinical assessments.
Handle all samples and reagents as potentially infectious and follow standard laboratory safety protocols.
Do not use the kit beyond its expiration date.
For in-vitro veterinary diagnostic use only.
The test must be administered and interpreted by a qualified veterinary professional.
A final diagnosis should not rely solely on the results of...
Other Information
Store the test kit at a temperature between 2°C and 8°C. Do not freeze. The kit is stable until the expiration date printed on the package when stored under the recommended conditions. Once opened, reagents should be handled according to the stability instructions in the package insert.
Store the test kit at a temperature between 2°C and 8°C. Do not freeze. The kit is stable until the expiration date printed on the package when stored under the recommended conditions. Once opened,...